DOT Testing Authorities

Federal Motor Carrier Safety Administration (FMCSA)

Federal Aviation Administration (FAA)

Federal Railroad Administration (FRA)

Federal Transit Administration (FTA)

Pipeline and Hazardous Materials Safety Administration (PHMSA)

United States Coast Guard (USCG)

Which Substances Are Tested?

DOT drug tests require laboratory testing for the following five classes of drugs:

Marijuana

Cocaine

Opioids

Amphetamines & Methamphetamines

Phencyclidine – PCP

The DOT testing at HHS-certified laboratories is a 5-panel drug test regimen.  As of January 1, 2018, the ‘Opiates’ category was renamed ‘Opioids’.

Under ‘Opioids’, previously ‘Opiates’, DOT testing will continue to include confirmatory testing, when appropriate, for Codeine, Morphine, and 6-AM (heroin).  The DOT added initial and confirmatory testing for the semi-synthetic opioids Hydrocodone, Hydromorphone, Oxycodone, and Oxymorphone to this Opioids group.  Some brand names for the semi-synthetic opioids include OxyContin®, Percodan®, Percocet®, Vicodin®, Lortab®, Norco®, Dilaudid®, Exalgo®.

Under Amphetamines, DOT testing includes confirmatory testing, when appropriate, for Amphetamine, Methamphetamine, MDMA, and MDA.  To this Amphetamines group, we added initial testing for MDA and removed testing for MDEA.

DOT Drug Testing
  • Requires specific collection procedures and certified laboratories
  • Includes testing for the trucking, maritime, aviation, public transportation, railroad, and pipeline industries